Digesting the DNA
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Revision as of 15:58, 16 June 2009 by 122.172.148.30 (talk) (Created page with ' Notes- There should not be too much salt in the digestion because too salt salt will inhibit enzyme activity. Observations: Plasmid Concentration 260/280 ...')
Notes- There should not be too much salt in the digestion because too salt salt will inhibit enzyme activity.
Observations:
Plasmid Concentration 260/280
IO500(P) 1790.5µg/µL 1.92 Lysis(L) 2324.4µg/µL 1.91
For Short Digestion
DNA = 2µL ECORI + Pst1 E1+E2 = 0.5µL + 0.5µL BSA = 2µL Buffer = 2µL MQ = 13µL
Total = 20µL
Time taken = 1.5 hours