DIY Plant Tissue culture and Engineering

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== DIY Designed Plant Tissue Culture ==

For the purpose of creating newly grown leaves from designed leaf cuttings, in-vitro

<a rel="license" href="http://creativecommons.org/licenses/by-nc-sa/3.0/us/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-sa/3.0/us/88x31.png" /></a>
DIY Designed Plant Tissue Culture by Allison Kudla is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-sa/3.0/us/">Creative Commons Attribution-Noncommercial-Share Alike 3.0 United States License</a>.


Materials and Equipment Needed

For making the plates:

Petri dishes

Erlenmeyer Flasks

Graduated Cylinders

Scale and Measuring Accessories

Magnetic Stirrer

Pipettes and Tips

Foil

Agar

Ionized water

Sucrose

Thiamine

Murashige + Skoog Packets

pH Meter

Autoclave

NAA (naphthaleneacetic acid)

BAP (benzyladenine)


For sterilizing the leaves:

Sterile Hood

Sterilized Water

Beakers

Bleach

Liquid Detergent (Household OK)

70% Alcohol

Flame

Forceps

Tweezers

Sterile gloves

Parafilm

Freshly Laser-cut or Die-cut Nicotiana Tabacum Leaves (still living)