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Revision as of 12:35, 23 November 2009
== DIY Designed Plant Tissue Culture ==
For the purpose of creating newly grown leaves from designed leaf cuttings, in-vitro
PLEASE NOTE:
<a rel="license" href="http://creativecommons.org/licenses/by-nc-sa/3.0/us/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-sa/3.0/us/88x31.png" /></a>
DIY Designed Plant Tissue Culture by Allison Kudla is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-sa/3.0/us/">Creative Commons Attribution-Noncommercial-Share Alike 3.0 </a>.
Materials and Equipment Needed
For making the plates:
Petri dishes
Erlenmeyer Flasks
Graduated Cylinders
Scale and Measuring Accessories
Magnetic Stirrer
Pipettes and Tips
Foil
Agar
Ionized water
Sucrose
Protocol
Thiamine
Murashige + Skoog Packets
pH Meter
Autoclave
NAA (naphthaleneacetic acid)
BAP (benzyladenine)
For sterilizing the leaves:
Sterile Hood
Sterilized Water
Empty Sterilized Petri Dishes
Beakers
Bleach
Liquid Detergent (Household OK)
70% Alcohol
Flame
Forceps
Tweezers
Sterile gloves
Parafilm
Freshly Laser-cut or Die-cut Nicotiana Tabacum Leaves (still living)