Difference between revisions of "ALS II"

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ALS II consists of 0.2M NaOH and 1% SDS(Sodium Dodecyl Suplhate).
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ALS II consists of 0.2M NaOH and 1% SDS (Sodium Dodecyl Suplhate).
 +
 
 
SDS is responsible for rupturing the cell membrane and NaOH raises the pH of <br>the media upto ph 14.0 at which the DNA can get denatured easily.
 
SDS is responsible for rupturing the cell membrane and NaOH raises the pH of <br>the media upto ph 14.0 at which the DNA can get denatured easily.

Revision as of 07:18, 12 June 2009

ALS II consists of 0.2M NaOH and 1% SDS (Sodium Dodecyl Suplhate).

SDS is responsible for rupturing the cell membrane and NaOH raises the pH of
the media upto ph 14.0 at which the DNA can get denatured easily.