Gluconacetobacter hansenii

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What is it?

the name of your traveling companion is Gluconacetobacter hansenii (cellulose strain).

Below is the media for optimal cellulose production, but we use it for culturing etc.

Isaac chile.jpg

Hestrin–Schramm (HS) medium (for 500mL)

  • 2.5g yeast extract (0.5% w/v)
  • 2.5g peptone (0.5% w/v)
  • 1.35g Na2HPO4 (0.27% w/v)
  • 0.75g citric acid (0.15% w/v)
  • 10g glucose (2%w/v) - NB autoclave this separately as 20% glucose, and add 50ml to 450ml of the rest of the media

other tips

I think is no problem with split the amount I gave you. To accomplish it, you should inoculate the media with a piece of the cellulose-bacteria layer and left it growing without agitation at room temperature (you only have to be careful with the contamination at the moment of inoculation!).

You can get a lot more information from this group of iGEM: http://2014.igem.org/Team:Imperial

If you are interested on dye the cellulose with bacterial pigments, we usually do this way:

  • grow the cellulose paper
  • remove the excess of media
  • use the cellulose paper as substrate, by plating (with L-shape spreader) a pigment producer O.N. bacterial culture over the wet cellulose paper.
  • Bacterias will grow on that side of the paper and produce the dye. If you want to have both sides dyed, repeat the process on the other side.

I hope it is clear :)

have a good time on Japan and don't hesitate to ask me if you need something.